Isolation of Flavonoid from Jackfruit Leaves (Artocarpus heterophyllus Lamk.) and its Antioxidant Activity

Abstract: The flavonoid compound was successfully isolated from jackfruit leaves (Artocarpus heterophyllus Lamk.). Extraction was done by maceration with methanol-water (9:1), followed by methanol-water (1:1) as a solvent. Hydrolyzed extract was obtained by adding of 2NHCl, then extracted with ethyl acetate and monitored by thin layer chromatography (TLC) with nhexane-ethyl acetate (3:5) as a mobile phase. Separation was performed by column chromatography, followed by preparativethin layer chromatography. Purity of isolates was determined by 2-dimensional thin layer chromatography. Characterization of isolate were carried out by ultraviolet-visible spectrophotometry and infrared spectrophotometry. The result showed a maximum absorbance of bands I at 328.4 nm and 271 nm for bands II. Characterization of isolate by infrared spectrophotometry showed the presence of functional groups OH, CH, C = C, C = O, CC, and CO. Based on the data, the isolate identified as a flavonoid compound. Antioxidant activity of ethyl acetate fraction and column fraction by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay showed EC50 at 60.53 mg/mL and 35.27 μg/mL respectively.
Key words: Artocarpus heterophyllus Lamk., flavonoid, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), EC50
Penulis: Soraya Riyanti, Clara Sunardi, Sana Nurul Falah
Kode Jurnal: jpfarmasidd130427

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