Antioxidant Activity and Antocyanin Derivatives Study of Red Katuk (Euphorbia cotinifolia L.) Leaves

Abstract: In this study, antioxidant activity and antocyanin derivatives study of extract of red katuk (Euphorbia cotinifolia L.) leaves was done. Antioxidant activity was done by DPPH (2,2-diphenyl 1-picrylhydrazyl) method using ultraviolet-visible spectrophotometer and thin layer chromatography. The result showed that methanol extract had antioxidant activity with IC50 value 33.13 μg/mL. Vitamin C as comparative substance had IC50 value was 5.24 μg/mL. From the acidified methanol extract, two anthocyanin derivatives have been isolated by preparative paper chromatography and characterized by ultraviolet visible spectrophotometry, which were supposed to be peonidin and cyanidin.
Keywords: Euphorbia cotinifolia L, antioxidant, antocyanin
Penulis: Ria Mariani, Iwang S. Soediro, Setiadi Ihsan, Sinta Eva, Desi Nuraisyah
Kode Jurnal: jpfarmasidd130428

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