EFEKTIVITAS PENGGUNAAN VOLTASE RENDAH PADA STERILISASI MEDIA BIAKAN BAKTERI
ABSTRACT: The important
treatment of culture media before being used is by sterilizing growth medium
first to avoid contamination from contaminant bacteria which can ruin the
result of bacteria in treatment ingredients. Nowadays, many researchers develop
new methods to sterilize, like; disinfecting microorganisms with
electrochemistry, electro-sterilization uses graphite electrode on liquid
culture media nutrient broth produces the decrease in bacteria rate by 16.7%..
This research tends experiment whose subject is culture media nutrient broth,
dependent variable is a number of dead bacteria and culture media nutrient
broth, independent variable is 8 electric potentials, 10 volts, and duration 5
until 10 minutes. Electro-sterilization treatment in subject is 4 with 6 times
repeat. Data collecting method use observation instruments of sterility test
result culture media nutrient broth. The result of data bivariate analysis uses
anova test determined that electro-sterilization will be effective and
efficient if it is done in 10 volts for 10 minutes. Data analysis univariate by
using frequent distribution determined that it is 8,3% of all total sample
contain dead bacteria. Analysis data uses correlation test determined that
culture media nutrient broth which has been given electro-sterilization
treatment function as culture media.
Keywords:
Electrosterilization, Electrode, Nutrient Broth
Penulis: Rodhiansyah
Djayasinga
Kode Jurnal: jpfarmasidd170058
