Antioxidant Activity of Combination Ethanol Extract of Turmeric Rhizome (Curcuma Domestica Val) and Ethanol Extract of Trengguli Bark (Cassia Fistula L) with DPPH Method
Abstrak: Turmeric rhizome
(Curcuma domestica Val) and Trengguli bark (Cassia fistula L) contain
antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl
(DPPH) free radical inhibition method. This research was conducted to determine
DPPH free radical inhibition by ethanol extract of turmeric rhizome, the
ethanol extract of trengguli bark, and a combination of turmeric rhizome
extract - trengguli bark extract (1:1,5) with ascorbic acid as a comparison.
Identification of secondary metabolite classes is performed by phytochemical
screening. Antioxidant activity was performed by inhibition of free radical
color of DPPH using UV-Vis spectrophotometry. The study showed IC50 value of
ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of
trengguli bark has the best antioxidant activity with IC50 value 10,98 μg/mL
compare to combination ethanol extract of turmeric rhizome - trengguli bark (1
:1,5) and ethanol extract of turmeric rhizome with IC50 value is 13,70 μg/mL
and 41,95 μg/mL, respectively.
Keywords: Antioxidant, Curcuma
domestica, Cassia fistula, DPPH Method
Penulis: Triyono, Anis Yohana
Chaerunisaa, Anas Subarnas
Kode Jurnal: jpfarmasidd180222
