TLC Profile and Activity Test of Secondary Metabolites Aspergillus flavus “In-Habiting” Queen Termite’s Nest Macrotermes gilvus on Enriched Media
Abstrak: Antibiotic are
secondary metabolites yielded by microbe especially fungus. Previous research
succesfull screened four kinds of fungus that live in termites queen’s nest,
one of them was Aspergillus flavus. Furthermore, Alen et al (2016g) states that
this fungus last to produce metabolite compounds on SDA media which only exist
in first and second subculture extract,
omit gradually for the next culture. It was presumed happen caused a different
habitat to grow. So it is necessary to enrich the media with queen termite nest
to get back the initial metabolites. The enrichment was done using four
different media concentrations (0.25; 0,50; 0.75 and 1 grams of nest/mL media).
The results show that enrichment of 1 gram of nest/mL media provides the most
optimum fungus growht. The third subculture of Aspergillus flavus is cultured
on enriched media which will become the fourth subculture, this fungus cultured
until tenth subculture, do extraction and
fracination to each culture. Based on TLC profile analysis, the initial
metabolite not yet formed until tenth subculture, but forms six new stain
terpenoid compounds. The result of columns chromatography obtained 10
sub-faction. Activity test was done by diffusion method to 12 test bacteria and 3 test fungus. Spot 2,5,6
(Rf 0.84; 0,36; 0,26) inhibit the growth of Pseudomonas aeruginosa ATCC 27853,
spot 3,4 (Rf 0.74; 0,52) inhibit the growth of Micrococcus luteus ATCC 10240.
The use of enriched media affect formation of
secondary metabolites Aspergillus flavus.
Keyword: Secondary
metabolites, Aspergillus flavus, Macrotermes gilvus Hagen., Enriched media, TLC
Profiles, Activity Assay
Penulis: Yohannes Alen, Rezki
Amelia, Akmal Djamaan
Kode Jurnal: jpfarmasidd180221
