MORPHOLOGICAL CHANGES OF CISPLATIN-RESISTANT HUMAN BREAST CANCER MCF-7 CELL LINE
ABSTRACT: To evaluate
morphological changes of breast cancer cell line MCF-7 following the
development of acquired resistance towards cisplatin. Resistance towards
anticancer agents still plays an important role in the breast cancer
chemotherapy failure.
Methods: The cisplatin resistant subline MCF-7/CisR was developed
in-vitro by cultivating the parental cell line cisplatin-sensitive MCF-7 with
raising concentration of cisplatin (from 0.01 to 2.3 µM) for four months. The
cell morphology was observed by giemsa staining.
Results: The resistance was shown by the increase of IC50 by 9 folds on
MCF-7/CisR compared to the initial IC50 value of MCF-7. The change in cisplatin
cytotoxic potency on the resistant cell line was accompanied by the
morphological modification, including the enlargement of cell size, the
increase of nucleus and cytoplasm ratio, and the increase of the number of
microvesicular and cytoplasmic granules.
Conclusions: This result has supported the underlying mechanism of
cisplatin resistance, including the ability of the cells to decrease
intracellular cisplatin concentration and repair DNA damage effects.
Keywords: Drug resistance,
human breast cancer, cisplatin, MCF-7
Penulis: Nanda Ayu Puspita,
Amy Bedford
Kode Jurnal: jpkedokterandd170220
