Examination of Telomerase Expression with Immuno-Hystochemistry Techniques on Some of Cancer Cells
Abstract: Cancer is a disease
that gets serious attention in the medical world. This is due to the ever
increasing number of patients and there has been no effective way to treat.
Cancer cells have telomerase activity is relatively high compared to normal
cells, so the cancer cells have the ability to continue to proliferate. Cancer
cells undergo uncontrolled mitosis and have high telomerase activity compared
to cells normal. Telomerase is an enzyme responsible for telomere length, a
segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres
are associated with the process of ageing and carcinogenesis. The purpose of
this study was to determine the expression of telomerase in some cells such as
breast cancer, cervical cancer, and lung cancer. Methods: The research method
is experimental studies in several cancer cell cultures in the form of cell
line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast
cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI,
DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal
cells). Expression of telomerase enzyme was measured by the
Immunohystochemistry (IHC) method. Results: The results showed that the cancer
cells have activity/higher telomerase expression were highly significant
(p<0.01) compared to normal cells (Vero cells). Similarly, the expression of
telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji
versus MCF7 also showed highly significant differences (p<0.01). Telomerase
expression between cancer cells that showed significant difference (HeLa cells
versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells)
(p<0.05). No significant difference was found in the group of HeLa cells
versus T47D, WiDr versus Raji cells, and WiDr versus MCF7. Conclusions: It was
concluded, that the cancer cells have telomerase expression of specific and
different from each other, depending on the type of cell. T47D breast cancer
cells have telomerase expression of the highest, followed by cervical cancer
cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the
same expression and both have lower expression.
Keywords: WiDr; HeLa; MCF-7
cells; telomerase; Immuno-hystochemistry
Author: Endang Purwaningsih,
Yulia Suciati, Etty Widayanti
Journal Code: jpkedokterangg160318
