Effects of Microencapsulated Synbiotic Administration at Different Dosages against heavy co-infection of White Spot Disease (WSD) and Vibrio harveyi in Pacific White Shrimp (Litopenaeus vannamei)
Abstract: White spot disease
(WSD) is one of infectious disease in shrimp caused by white spot syndrome
virus (WSSV). This study aimed to determine the dosage immunological effects
and growth performances of microencapsulated synbiotic (Bacillus NP5 and mannan
oligosaccharide) at different dosages on Pacific white shrimp. The microencapsulated synbiotic was administered as feed
supplementation against the co-infection
of WSSV and Vibrio harveyi. Synbiotic
was encapsulated by spray drying method, further feed supplemented to Pacific
white shrimp for 30 days at a dosages of
0.5% (A), 1% (B), 2% (C) and control treatments, i.e. without any
microencapsulated synbiotic administration as positive control (D) and negative
control (E). The challenge test was performed on day 30 after feeding
supplementation, then the experimental shrimps were injected by WSSV
intramuscularly at the infective dosage
of 104 copies.-ml-1. Afterwards, 24
hours after WSSV injection the shrimps were immersed in water contained cells
suspension of V. harveyi at the cells
population dosage of 106 CFU-.ml-1. All synbiotic treatments showed better
results with the values of Total Haemocyte Count (THC), Phenoloxidase (PO) and
Respiratory Burst (RB), were higher (P<0.05) compared to positive control.
The specific growth rates (SGR) of A, B and C showed higher than both controls
of D and E. The feed conversion ratio (FCR) value of synbiotic treatments were
lower (P<0.05) than both controls. However, the administration of
microencapsulated synbiotic have not been able to prevent heavy impact of WSSV
and V. harveyi co-infection due to lower SR and mortality pattern which
continued to increase.
Keywords: Synbiotic,
Litopenaeus vannamei, WSSV, Vibrio harveyi, co-infection
Author: Yunarty, Munti Yuhana,
Widanarni
Journal Code: jpperikanangg160066
