Screening of Antibacterial MDR derived from Sponge Associated Fungus of Riung Water, Nusa Tenggara Timur
Abstract: Marine
sponge-associated fungi are the sources of bioactive compounds with various
pharmacologicals potency. This study aimed to isolate the sponge-associated
fungi as the producer of the MDR anti-bacterial compounds. The associated fungi were isolated from the
sponges collected from Riung water, Nusa Tenggara Timur. Five of the best
isolates were cultured on MEA to obtain the methanolic extract for further
studies. The antagonistic test was
conducted using overlay method towards the MDR Staphylococcus aureus and
Escherichia coli. A total of 33 fungi were isolated from 19 sponge specimens.
The antagonistic test showed that 19 isolates were active against both S.
aureus and E. coli, and 13 of them were merely active against one of the
bacteria. However, only five isolates have strong activity against one or both
of the bacteria. The KN-15-3 had the
strongest activity against S. aureus (18.75±0.777mm) and E. coli
(15.10±0.141mm) at the concentration of 400 μg.disc-1 so it can be developed
further as a source of drug candicate.
Keywords: Fungi symbiont,
Sponges, MDR Antibacterial, Staphylococcus aureus, Escherichia coli
Author: Khoeruddin
Wittriansyah, Agus Trianto, Sekar Widyaningsih, Ocky Karna Radjasa, Rudhi
Pribadi
Journal Code: jpperikanangg160067
