Development of Sybr Green I-Based Melting Curve Method for HER2I655V Polymorphism Detection in Breast Cancer
Abstract: Currently available
molecular method to detect HER2I655V polymorphism such as PCR-RFLP is hampered
by the costly experimental method, and post-PCR treatment requirement that
makes this technique is not meeting for high-throughput analysis purpose. In
this study, we developed an accurate, simple, low cost and rapid test to detect
polymorphism at HER2 gene using SBR Green I based-melting curve method.
Methods: Two forward allele-specific primers and one common reverse primer were
used then these primers were tested to discriminate known genotypes of genomic
templates (GG type or AA type) and genomic samples retrieved from breast cancer
patients. Results: Melting curve analysis derived from SYBR Green I-based
allele-specific PCR with defined primers concentration and annealing
temperature at 54.3 °C showed good discrimination level of Tm peaks in which GG
genotype melted at 89 °C slightly higher than AA genotype which melted at 86
°C, while AG genotype harbored both of homozygous Tm characteristics. Conclusions:
This preliminary result will be as basic for further large-scale typing of
HER2I655V polymorphism.
Keywords: breast cancer; HER2;
polymerase chain reaction; polymorphism; SYBR Green I
Author: Bugi R Budiarto,
Wirsma A Harahap, Desriani Desriani
Journal Code: jpkedokterangg160321
