Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines
Abstract: Garcinia
xanthochymus extract has been reported to have several pharmacological
properties. This study was conducted to evaluate cytotoxic and
anti-inflammatory activities of G. xanthochymus extracts on cell lines.
Methods: The roots and stem barks of plant were extracted using maceration
method with n-hexane, dichloromethane and methanol, successively. Cytotoxic
activity of the extracts was tested against MCF-7 breast adenocarcinoma using
MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse
macrophage cells. The nitric oxide production in LPS-stimulated cells was
measured using Griess reagent. Results: The results of cytotoxic and
anti-inflammatory study showed that dichloromethane and n-hexane extracts of
root and stem bark exhibited cytotoxic activity in dose-dependent manner.
Meanwhile, for anti-inflammatory study, all root extracts together with stem
bark dichloromethane and n-hexane extracts reduce NO production in
LPS-stimulated cells in dose dependent manner. Conclusions: This finding
indicated that G. xanthochymus extracts might become interesting candidate for
treatment of cancer and inflammation.
Keywords: Garcinia
xanthochymus; Guttiferae; cytotoxicity; anti-inflammatory; in vitro
Author: Hanisuhana Hamidon,
Muhammad Taher, Juliana M Jaffri, Tg M Tg Zakaria, Wan MAW Sulaiman, Deny
Susanti, Solachuddin JA Ichwan, Zainul A Zakaria
Journal Code: jpkedokterangg160323
