Catechins decrease neurological severity score through apoptosis and neurotropic factor pathway in rat traumatic brain injury
Abstract: Catechins inhibits
apoptosis through anti oxidant and anti inflamation pathway. Catechins also
increases brain-derived neurotrophic factor (BDNF). There was a few research
that explained the role of catechins in traumatic brain injury (TBI). The
objective of the study was to evaluate the effect of catechins administration
on neurologic severity score (NSS) through apoptosis and neurotropic pathway in
traumatic brain injury rat model.
METHODS
A post test only controlled group design was performed using traumatic
brain injury rat (Rattus novergicus) model through weight drop models. It was
devided into negative control group, positive control group, TBI+catechins 513
mg/kgBW, TBI+catechins 926 mg/kgBW, TBI+catechins 1113 mg/kgBW. NSS was
measured in the first hours, day three, and day seven. The expressions of NFkB,
TNFa, Bcl-2, Bax, caspase 3, caspase 8, BDNF, and the numbers of apoptosis
cells were evaluated by imunohistochemystry method. One way Anova and Kruskal
Wwallis were used to analyse the data.
Results
TNFa, caspase 8, number of apoptosis cells were significantly decreased
on the seventh day administration compared to the third day administration
(p<0.05). Catechins increased the expression of Bcl-2/Bax and BDNF significantly
(p<0.05). Yet, there were no significant differences between expression of
caspase 3, NSS, Bcl-2/Bax ratio, and BDNF toward third days administration of
catechins compared with seven days administration (p>0.050).
Conclusions
Administration of catechins decreased NSS through inhibiting inflammation
and apoptosis, as well as induced the neurotrophic factors in rat brain injury.
Catechins may serve as a potential intervention for TBI.
Keywords: Catechins; traumatic
brain injury; apoptosis; inflammation; rats
Author: Retty Ratnawati,
Annisa Nurul Arofah, Anastasia Novitasari, Sartika Dewi Utami, Made Ayu
Hariningsih, Masruroh Rahayu, Sri Budhi Rianawati, Hari Purnomo, Mochammad
Dalhar
Journal Code: jpkedokterangg170037
