Aloe vera extract reduces 8-oxo-2'-deoxyguanosine levels and improves total antioxidants in streptozotocin-induced diabetic rats
Abstract: Diabetes mellitus is
a chronic disease caused by lack of insulin production in the pancreas or by
insulin resistance, the disease being characterized by elevated blood glucose
levels. Hyperglycemia in diabetes could lead to oxidative stress due to the
rise in 8-oxo-2'-deoxyguanosine (8-oxo-dG) levels and the decrease in levels of
total antioxidant status (TAS). The purpose of this study was to assess the
effect Aloe vera extract on 8-oxo-dG level and total antioxidant status in
diabetic rat testis.
Methods
This was an experimental laboratory study with 20 rat samples which were
divided into 4 groups (1 control group and 3 treatment groups). Diabetes was
induced in the rats by streptozotocin (STZ) at 65 mg/kgBW. The diabetic rats
were then treated for 28 days with Aloe vera extract at 0 mg (P0), 200 mg rind
(P1), 200 mg pulp (P2), respectively. The level of 8 -oxo-dG was measured by
ELISA and total antioxidant status with 2,2' -azino-bis
(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Data were analyzed with ANOVA
and Least Significant Difference Advanced Test at P<0.05.
Results
8-Oxo-dG levels were significantly different between the control group
and both P0 and P2, but not between the control group and P1. Among the
treatment groups the 8-oxo-dG levels were significantly different. Mean total
antioxidant status was significantly different between control and treatment
groups, and also between treatment groups (p<0.05).
Conclusions
Aloe vera extract reduced free radicals (level of 8-oxo-dG) and increased
the total antioxidant status in diabetic rat testis.
Keywords: Aloe vera; 8-oxo-dG;
total antioxidants; diabetes mellitus
Author: Wulan Christijanti,
Aditya Marianti, Wiwi Isnaeni
Journal Code: jpkedokterangg170036
