AUTHENTICATION OF RAW CHICKEN MEAT FROM PORK CONTAMINATION USING GENE CYT-B WITH DUPLEX-POLYMERASE CHAIN REACTION ANALYSIS
ABSTRACT: Halal is one of
important aspects in consumer protection. Meat and processed meat products are food
that should be controlled strictly because those are prone to be adulterated by
pork contamination. Therefore, it is necessary to provide detection technique
which is accurate, fast and cheap. The objective of this research was to
identify the presence of impurities of pork meat on raw chicken meat using gene
Cyt-b with duplex-PCR analysis. This research used six samples of raw chicken
meat and raw pork. Raw chicken meat was bought from supermarkets in the city of
Surakarta and raw pork was obtained from pigslaughterhouse. The percentage of
raw pork contamination on raw chicken meat was designed as much as1, 5, 10, and
25%, respectively. The DNA genome was isolated according to DNA isolation
protocol fromGenomic DNA Mini Kit. In addition, duplex-PCR was performed based
on protocol of KAPA2G Fast Multiplex PCR kit. The data was descriptively
analyzed by directly looking the DNA bands on the gel documentation apparatus.
The result showed that specific DNA bands for chicken and pig were completely appeared
on 1.5% of agarose gels. Duplex-PCR detect contamination of pork on raw meat of
chicken at all contamination levels. This research proved that the duplex-PCR
detect the contamination of pork until the level of 1%.
Keywords: Authentication,
Chicken meat, Cytochrome b, Duplex-PCR, Pork contamination
Penulis: Bayu Setya Hertanto,
Rizky Aulia Fitra, Lilik Retna Kartikasari, and Muhammad Cahyadi
Kode Jurnal: jppeternakandd170169