Screening of Indonesian Streptomyces sp. Capable of Secreting Transglutaminase (Mtgase) and Optimization of Mtgase Production Using Different Growth Media
Abstract: Transglutaminase
(TGase), an enzyme that catalyzes the formation of inter- and intra-molecular
e-(l-glutamyl) lysine (GL) crosslinks, plays an important role in surimi-based
products production. The development and the diversification of surimi-based
products have recently been getting popular in Indonesia. These surimi-based
products can be made from various types of fish. These products generally
exhibit good gel strength properties,
depending on the fish type and the processing method used.
Transglutaminase plays an important role in generating such properties. Fish’s
endogenous TGase reduces quickly after it is caught and is almost completely
destroyed by freezing it, applying exogenous TGase may improve fish’s gel
forming ability. Microbial transglutaminase (MTGase) can potentially be used to
increase gel properties. In this research, a total of 228 Streptomyces strains
from marine and terrestrial environments were screened and selected based on
their ability to produce MTGase. Strain TTA 02 SDS 14 exhibited the highest
activity; and therefore, it was selected for further study. The 16S-rRNA gene
analysis showed that it shared 99% similarity to S. thioluteus. In order to
optimize MTGase activity, enzyme production was carried out using six different
formulas media, designated as media A, B, C, D, E, and F. The result shows that
the highest MTGase activity was observed in medium B that contains pepton
(1.5%), MgSO4.7H20 (0.1%), KH2PO4 (0.5%), Na2HPO4 (0.5%), soybean powder (2%),
potato starch (2%), and glucose (1.5%). The MTGase activity reached the highest
level (1.45 U/ml) after 4 days of incubation
Keywords: screening,
transglutaminase, Streptomyces, media composition
Author: Yusro Nuri Fawzya,
Dewi Seswita Zilda, Seprianto Chaniago, Hana Nurullita Prestisia, Puspita
Lisdiyanti, Noer Khasanah
Journal Code: jpperikanangg160028
