The Optimal Conditions of Xylanase Production Using Empty Fruit Bunch Raw Biomass by Marine Isolate LBF-001
Abstract: Several xylanases
have already been studied. However, only a few xylanases derived from marine
microorganisms has been reported. Marine bacterium LBF-001 was isolated from
Pari Island Kepulauan Seribu, Indonesia. The purpose of this study is to
identify isolate LBF-001 using 16S rDNA gene and to optimise the medium
conditions for the xylanase production i.e. concentration of biomass, nitrogen
source, pH and temperature. Based on 16S rDNA gene analyses, LBF-001 isolate
has 99% similarity with Bacillus pumilus HNS70 (KF933667). Fermentation process
to produce xylanases was conducted using several agricultural residues under
solid-state fermentation (SSF). The optimum condition for xylanase production
by B. pumilus LBF-001 was using a medium containing 2.5% empty fruit bunch and 0.6% lactose
broth, at pH 6.5, temperature 30 oC, under submerged fermentation with shaking
at 150 rpm for 48 h fermentation. The optimised condition resulted higher
xylanase activity, i.e. 10.85 U/mL.
Keywords: empty fruit bunch,
marine bacteria, optimisation, submerged fermentation, xylanase
Author: Hans Wijaya, Ahmad
Thontowi, Nanik Rahmani, Yopi Yopi
Journal Code: jpperikanangg160029
