Cytotoxic Activity and Secondary Metabolite Characteristics of Sea Cucumber Actinopyga sp. Methanolic Extract
Abstract: The objectives of
this research were to assess cytotoxic activity and examine characteristic of sea
cucumber Actinopyga sp. methanolic extract. Cytotoxicity assay was conducted
using MTT method against WiDr (colon cancer) and T47D (breast cancer) cell
lines. Actinopyga sp. methanolic extract was characterized by using
phytochemical screening, Transform-Infrared (FT-IR) spectroscopy, dan Liquid Chromatography Ion Trap Time of Flight
Mass Spectrophotometer (LC-IT-ToF-MS).
The result showed that Actinopyga sp. methanolic extract inhibited Widr
and T47D cell lines viability with the IC50 value of 55.93 and 87.55 µg/mL,
respectively. Functional groups analysis showed the presence of hydroxyl,
amine, carboxylic acid, nitrate, amide, sulphur, ester, and ether. LC-IT-ToF-MS
analysis of crude extract showed that it containing many steroids compound.
Keywords: Actinopyga sp.,
secondary metabolite characteristic, cytotoxic, WiDr, T47D
Author: Muhammad Nursid,
Afrilia Putri Maharani, Riyanti Riyanti, Endar Marraskuranto
Journal Code: jpperikanangg160027
