Cloning of a Transglutaminase Gene from an Indonesian Streptomyces Strain
Abstract: Microbial
Transglutaminase (MTGase, EC 2.3.2.13) is an enzyme that catalyzes the transfer
of acyl group. Many microbial strains that produce MTGase belong to
Streptomyces members. This research was aimed at cloning of a MTGase gene.
PCR–based screening of ten MTGase-producing streptomyces isolates from soil in
West Nusa Tenggara led to detection of one potential isolate, designated as TTA
02 SDS 14. The partial MTGase-encoding
gene (470 bp) was amplified by PCR and
sequenced. The sequence result indicate its similarity of 93 % with that of
Streptomyces cinnamoneus. The 16S rRNA gene analysis showed its identity as
Streptomyces thioleteus. Fosmid-based construction of a genomic library from
the isolate and subsequent screening led
to the isolation of a ~40-Kb fosmid
harboring a MTGase gene.
Keywords: MTGase gene,
Streptomyces spp, genom library
Author: Seprianto Chaniago
Journal Code: jpperikanangg160026
