PENGGUNAAN RAGI ROTI (SACCHAROMYCES CEREVISIAE) SEBAGAI IMUNOSTIMULAN UNTUK MENINGKATKAN RESISTENSI IKAN MAS (CYPRINUS CARPIO L) TERHADAP INFEKSI BAKTERI AEROMONAS HYDROPHILA
ABSTRACT: A research had been
conducted to evaluate the use of baker’s yeast as immunostimulant in enhancing
the resistance of carp (Cyprinus carpio L) to Aeromonas hydrophila
infection. Experimental fish were
obtained from Freshwater Aquaculture Board in Talelu, Minahasa Regency, and
transported to the Laboratory of Aquaculture Technology at the Faculty of
Fisheries and Marine Science, Sam Ratulangi University. During two weeks of acclimatization process, fingerlings were fed
with commercial feed (pellet) at 5% of body weight per day, twice a day. After acclimatization, fish was randomly
distributed into 15 aquaria with a density of 25 fishes/aquarium. The doses of
yeast cells as treatments included 5, 10, 15 and 20 g yeast/kg of pellet while
control pellet not supplemented with yeast cell. Fish was fed with treatment
pellets for four consecutive weeks at 5% of body weight per day, twice daily at 09.00 am and 16.00 pm.
Afterward, the density of fish were adjusted to 10 fish/aquarium. Fish were
then challenged with A. hydrophila through intraperitoneal injection of 0.2 mL of A. hydrophila suspension containing
1x107 cfu/mL. Research result showed that supplementation of yeast cells into
fish pellet significantly influenced the resistance of fish to A. hydrophila
infection (p<0,01). The highest resistance were observed in fish fed pellet
supplemented with 5 g yeast/kg of pellet. Thus, supplementation of baker’s yeast cells into commercial fish feed
was able to increase resistance of carp
to A. hydrophila infection.
Keywords: baker’s yeast,
Cyprinus carpio, A. hydrophila, resistance, intraperitoneal injection
Penulis: Henky Manoppo,
Magdalena E.F. Kolopita
Kode Jurnal: jpperikanandd160656
