IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT
Abstract: The development of
micropropagule and embryo of seaweed depend on nutrient and fertilizer used.
Seaweed has been reported contain hormone regulators such as auxine,
cytokinine, gibbereline, and various minerals applied in stimulating the growth
ocra plant and wheat culture. The objectives of this study were to determine
the potential of Kappaphycus alvarezii extract and its optimal concentration in
accelerating of Kappaphycus alvarezii micropropagule and embryo growth.
Micropropagule and embryo produced through callus induction were planted into
PES 1/20 liquid medium supplemented with seaweed extract at the concentrations
of 0 (control), 25, 50, 75, and 100 μL in 10 mL of medium. The results showed
that medium enrichment with 50 μL of seaweed extract had the highest survival
rate and growth of thallus. In addition, this concentration was also resulted
in a good performance of K. alvarezii thallus with the lighter color. The
advantage of this study for seaweed cultivation in Indonesia, among others,
seaweed can be used as fertilizer, especially in the maintenance of seaweed
seed, so that cultivation can be better develop.
Keywords: callus induction;
filamentous callus; micropropagule; embryo; Kappaphycus alvarezii
Author: Emma Suryati, Rosmiati
Rosmiati, Andi Parenrengi, Andi Tenriulo
Journal Code: jpperikanangg150029
