Effect of Agitation Speed and Cultivation Time on the Production of the Emestrin Produced by Emericella nidulans Marine Fungal
Abstract: Emestrin, an
epipolythidioxopiperazine (EPT), is bioactive secondary metabolite produced by
the marine fungus Emericella nidulans. Emestrin is potential to be developed as
anticancer agent. Our present study investigated the effect of the agitation
speed and cultivation time on the production of the mycelial biomass and
emestrin in E. nidulans. The fungal was cultivated in malt extract broth (MEB)
medium with varying agitation speeds of 0, 50, 100,150 rpm during 1,2,3 and 4
weeks of incubation at the temperature of 28 oC. Concentration of emestrin was
determined by using high performed liquid chromatography (HPLC). The highest
concentration of emestrin was found at static condition (0 rpm) cultivated for
1 week.
Keywords: emericella
nidulands, emestrin, agitation speed, culvation time
Author: muhammad nursid
nursid, Martua Manulang, Joko Samiadji, Endar Marraskuranto
Journal Code: jpperikanangg150030
