ASSOCIATION OF MICROSATELLITE GENETIC DIVERSITY WITH GROWTH RELATED TRAITS IN THE BASE POPULATION OF AFRICAN CATFISH, Clarias gariepinus, BREEDING PROGRAM
Abstract: Genetic diversity at
molecular level has been assumed to correlate with fitness related traits.
However, accumulating evidences showed that the nature of that correlation has
been variable. This study was aimed to explore the nature and possible mechanisms
underlying that correlation by focusing on growth related traits in African
catfish, Clarias gariepinus using microsatellite molecular markers. Fifty
individual African catfish of 110 days-old were sampled and subjected to both
morphological and molecular analyses. The standard length, total length and
body weight as well as allelic scores of six microsatellite loci were measured
on each individual. Indices of microsatellite diversity, namely individual
multilocus heterozygosity (MLH) and mean microsatellite allelic distance (mean
d2) for individual level, and mean observed heterozygosity (Ho) and
single-locus heterozygosity (ho) for group level, were correlated to those
traits using Pearson correlation coefficient (r). The Hardy Weinberg and
linkage disequilibrium were carried out to explore the possible mechanisms
underlying correlation. The results showed that at individual level the MLH and
mean d2 were weakly correlated with standard length, (r=0.25, p<0.05) and
(r=0.24, p<0.05), respectively. At group level, Ho was correlated with both
standard and total length (r=0.99, p<0.05) while ho identified two loci,
Cga03 and Cga06 significantly contributed to the correlation. Combining all
relevant information, present study identified associative overdominance, both
local effect and general effect hypotheses might responsible for the observed
correlations.
Keywords: microsatellite
genetic diversity; multilocus heterozygosity; microsatellite allelic distance;
observed heterozygosity; African catfish; Clarias gariepinus
Author: Imron, Bambang
Iswanto, Narita Syawalia Ridzwan, Rommy Suprapto, Huria Marnis
Journal Code: jpperikanangg150028
