DETECTION, TRANSMISSION, AND EXPRESSION cDNA GROWTH HORMONE GENE (PhGH) OF STRIPPED CATFISH IN F-1 TRANSGENIC AFRICAN CATFISH
Abstract: In previous study,
the fast growth transgenic founder of African catfish was produced harboring a
growth hormone (GH) gene construct containing a stripped catfish growth hormone
(PhGH) cDNA. This study was conducted to investigate transgene (PhGH)
transmission and expression in F-1 transgenic African catfish. The transgenic
founders (female) were crossed with non-transgenic (male) to produce
heterozygous F-1 progeny. PhGH gene was detected in the embryo, larvae, and
seed of the transgenic F-1 using PCR method. Expression levels of transgene in
embryo and larvae were analyzed using real-time quantitative PCR (qPCR) method.
The transgene was detected in embryo, larvae and seed of F-1 transgenic African
catfish. Founder could transmit PhGH gene to transgenic F-1 lines in ranged 36%
to 48%. Expression level of Phgh gene in embryo was higher than that of the
larvae; whereas in the embryo was 1.5 x 105 - 5.2 x 105 copies or 0.49-9.82
fold, while in the larvae was 1.1 x 105 - 2.5 x 105 copies or 0.19-5.80 fold.
Keywords: African catfish;
transgenic; growth hormone; transmission; gene expression
Author: Huria Marnis, Bambang
Iswanto, Rommy Suprapto, Imron, Raden Roro Sri Pudji Sinarni Dewi
Journal Code: jpperikanangg140046
