DETECTION OF Macrobrachium rosenbergii Nodavirus (MrNV) AND EXTRA SMALL VIRUS (XSV) DISEASES ON GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii AT SAMAS, YOGYAKARTA
Abstract: Mass mortality of
giant freshwater prawn (Macrobrachium rosenbergii de Man) in grow-out farmers
occurred in early February 2012 at Instalation Coastal of Aquaculture Samas,
Bantul, D.I. Yogyakarta. The clinical sign of shrimp was whitish coloration on
abdominal and tail muscle. The sympton was the same as in other cases of
mortality caused by prawn Macrobrachium rosenbergii nodavirus (MrNV) and Extra
Small Virus (XSV). Prawn samples were diagnosed by standard protocols Reverse
Transcriptase-Polymerase Chain Reaction (RT-PCR) using specific primers and histopathology
analysis. The result showed that all samples indicated positive 13/15 the MrNV
and 5/15 positive XSV, and there were 4/15 positive samples both (MrNV and
XSV). Analysis of histopathology showed that damaged muscle was indicated by
the presentation of necrotic tissues with nuclear pyknosis or degeneration of
muscle in infected tissues. Based on diagnosis by RT-PCR and histopathological,
mass mortality of the giant freshwater prawn in Indonesia is determined to be
caused by “white muscle disease (WMD)/white tail disease (WTD)”.
Keywords: freshwater prawn;
Macrobrachium rosenbergii nodavirus (MrNV) and Extra Small Virus (XSV); RT-PCR;
histopathology
Author: Isti Koesharyani, Lila
Gardenia
Journal Code: jpperikanangg140060
