DEVELOPMENT OF PRIMARY CELL CULTURE FROM TAIL EPIDERMAL TISSUE OF KOI CARP (Cyprinus carpio koi)
Abstract: Primary cell culture
from tail epidermal tissue of koi carp (Cyprinus carpio koi) was developed.
Cells were grown in Leibovits-15 medium supplemented with 20% fetal bovine
serum and antibiotics (Penicillin/Streptomycin and Kanamycin). Cell growth was
observed in a range of incubation temperature (17oC±2oC, 22oC±2oC, 27oC±2oC,
and 32oC±2oC) in order to determine the optimum temperature. The cells were
able to grow at a range of temperature between 17oC to 32oC with optimal growth
at 22oC. Primary cells infected with koi herpes virus produced typical
cytopathic effects characterized by severe vacuolation and deformation of
nuclei, which is consistent with those of previous reports. Artificial
injection experiment by using supernatant koi herpes virus SKBM-1 isolate
revealed that it could cause 90% mortality in infected fish within two weeks.
PCR test with Sph I-5 specific primers carried out with DNA template from
supernatant virus, pellet cell, and gills of infected fish showed positive
results in all samples (molecular weight of DNA target 290 bp). The cells were
found to be susceptible to koi herpes virus and can be used for virus
propagation.
Keywords: koi herpes virus;
primary cell culture; koi fish
Author: Lila Gardenia, Isti
Koesharyani
Journal Code: jpperikanangg140061
