ANALYSIS OF IMMUNE RESPONSES ON TRANSGENIC TIGER SHRIMP (Penaeus monodon) AGAINST PATHOGENIC BACTERIUM Vibrio harveyi
Abstract: Vibriosis is one of
main diseases of the black tiger shrimp Penaeus monodon infected by pathogenic
bioluminous bacterium Vibrio harveyi that can cause mass mortalities in shrimp
culture. The bacteria can also trigger the disease white spot syndrome virus
(WSSV). An effort to produce shrimp disease-resistant strains has been done
through transgenesis technology with antiviral gene transfection. By this
technology, it is expected an increase in the immune response of shrimp in a
variety of diseasecausing pathogens. This study aimed to determine the immune
responses (total haemocytes, haemocyte differentiation, and phenoloxydase
activity) of transgenic tiger shrimp against pathogenic bacterium V. harveyi.
Research using completely randomized design, which consists of two treatments
and three replications. Test animals being used were transgenic and
non-transgenic shrimp with size, weight 3.93±1.25 g and a total length of
7.59±0.87 cm. Treatments being tested were the injection of bacterium V.
harveyi (density of 5x106 cfu/mL) of 0.1 mL/individual on transgenic (A) and
non-transgenic shrimp (B). Immune response parameters such as total haemocytes,
haemocyte differentiation, and phenoloxydase activity were observed on day 1,
3, and 6 days after challenging. Data were analyzed using t-test by SPSS
software. The results showed that the total haemocyte of transgenic shrimp was
not significantly different (P>0.05) from non-transgenic shrimp, but
haemocyte differentiation and phenoloxydase activity were significantly
different (P<0.05) especially on sixth days after being exposed to the
bioluminescent bacteria. The study results implied that transgenic shrimp has a
better immune response compared than non-transgenic shrimp.
Keywords: immune response;
transgenic; tiger shrimp; bacteria; Vibrio harveyi
Author: Andi Parenrengi, Bunga
Rante Tampangallo, Andi Tenriulo
Journal Code: jpperikanangg140059
