DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION
Abstract: Chitooligosaccharide
(COS) , which is a derivative product from chitosan, has recently been used as
a functional food because it has antimicrobial, antifungal, and antitumor
properties. The salt content in chitooligosaccharide is one of the main
problems in application as functional food or pharmaceutical medicine. The aim
of this study was to remove salt from COS with two desalting techniques and
determine the variation of COSs in the product. The desalting technique used
were dialysis with 10kD Molecular Weight Cut Off (MWCO) and gel filtration
chromatography HiPrep 26/10 desalting with G-25 Superfine Sephadex as
stationary phase in the column. In order to detect the presence of COS, Thin
Layer Chromatography (TLC) method was used, followed by Matrix-Assisted Laser
Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI–TOF–MS) to detect
low concentration of COS. Qualitative and quantitative analysis of salt
presence were identified using silver nitrate and Volhard method respectively.
Ash content was measured using gravimetric method. Results showed those
dialysis and gel filtration chromatographies were successfully remove the most
of the salt from COS with efficiency of desalting up to 100%. However, the best
desalting technique was gel filtration chromatography HiPrep 26/10 which has
more complete COS with various degrees of polymerization present in the result.
Keywords: Chitooligosaccharide,
desalting technique, dialysis, gel filtration chromatography
Author: Pujoyuwono
Martosuyono, Asri Pratitis, Alexander Prasetya, Elisabeth Kartika Prabawati
Journal Code: jpperikanangg140044
