IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STAR FISH (ACANTHASTER PLANCI) ON LYMPHOCYTE PROLIFERATION IN-VITRO
Abstract: The aims of this
study were to investigate lymphocyte proliferation activity and to identify
chemical constituents of active fractions of star fish Acanthaster planci. A.
planci was collected from Ternate Island, North Moluccas, extracted with
distilled methanol and water, partitioned with gradient
chloroform-hexane-methanol-water and fractionated in column chromatography
using silica gel and hexane-ethyl acetate-methanol. The active compound had
been purified by Thin Layer Chromatography (TLC) and identified by Gas
Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity
was measured based on % Stimulation Index (SI) from sample absorbency and
control absorbency. The result showed that the 3 fractions of hexane fraction
exhibited lymphocyte proliferation activity. Fraction 1 was able to increase
lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88%
(160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml)
increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml)
increase. Metabolite analysis of active fraction using GC-MS yielded a number
of chemical constituents that was dominated by fatty acid. The study concluded
that star fish A. planci from Ternate Island has a potential source of
immunostimulator.
Keywords: Acanthaster planci,
immunostimulatory, lymphocyte proliferation
Author: M. Janib Achmad, Alim
Isnansetyo, Noer Kasanah, Ustadi Ustadi, Kamiso Kamiso
Journal Code: jpperikanangg140043
