CLONING OF ProAV PROMOTER ISOLATED FROM TIGER PRAWN Penaeus monodon
Abstract: Promoter is a
specific DNA sequence involved in the transcription of a particular gene. It is
usually located in the upstream of the gene they regulate. Isolation and
characterization of promoter is essentially needed in order to establish the
sequence analysis and transcription factor that are used in the regulation of
gene expression. The research was conducted to analyze the characteristics of
Penaeus monodon anti viral gene promoter (ProAV) towards generation of
auto-transgenic tiger prawn, P. monodon. ProAV promoter was isolated by PCR
(Polymerase Chain Reaction) method and the purified DNA fragment was cloned
into pGEM-T Easy cloning vector. The promoter sequence was characterized by
using BLAST-N and Genetyx version 7 softwares. The results showed the success
in isolating a promoter from tiger prawn of 368 bp in length. BLAST-N analysis
showed that the sequence of isolated promoter has high similarity (95%-98%)
compared to the other promoters in the GeneBank. The study revealed the
existence of important transcription factors (TATA box, MRE, TCF-1, and other
potential regulatory elements) are identified in the promoter sequence.
Keywords: promoter; cloning;
DNA sequence; tiger prawn
Author: Andi Parenrengi,
Alimuddin, Sukenda, Komar Sumantadinata, Muhammad Yamin, Andi Tenriulo
Journal Code: jpperikanangg090016
