THE EFFECTS OF STORAGE TEMPERATURE AND TYPES OF MEDIA ON THE SURVIVAL RATE OF S. agalactiae BACTERIOPHAGE
Abstract: The successful
effect of phages in phage therapy has been proven by an increase of the
presence of phages in survivors. Seven previously isolated phages were used to
examine the effects of storage temperature and types of media on their survival
rate. Phages PSaT-18, PSaT-19, PSaT-20 and PSaT-21 isolated from a
Streptococcosis-infected Tilapia (Oreochromis niloticus) were used in this
study. Phages PSaG-1, PSaG-2 and PSaG-3 were isolated from a diseased Gouramy
(Osphronemus gouramy). All of the phages were stored in refrigerator at
temperature of 8oC and kept in two types of media, Brain Heart Infusion Broth
(BHIB) and BHIB enriched with glycerol. To determine the lytic activity of the
phages, 20 μL of each phage suspension was poured onto BHIA plate and incubated
at 25oC for 24 to 48 hours. The result showed that phages PSaT-18, PSaT-19,
PSaT-20 and PSaT-21 have lytic activity against S. agalactiae and S. iniae than
phages PSaG-1, PSaG-2, and PSaG-3. Phages PSaT-18, PSaT-19, PSaT-20, and
PSaT-21 always survived up to three weeks and phage PSaT-18 in BHIB have the
highest potential as a candidate of phage therapy for Streptococcosis.
Keywords: storage temperature;
types of media; survival rate; bacteriophage
Author: Angela Mariana
Lusiastuti, Tuti Sumiati, Adin Priadi
Jurnal Code: jpperikanangg080016
