Bioassay-Guided Isolation of an Antibacterial Compound from the Indonesian Soft Coral
Abstract: With the aim of
searching for new antibacterial compound from marine soft corals, the
investigation had been conducted on antibacterial activity of an extract from
soft corals Sarcophyton trocheliophorum in the n-hexane, ethyl acetate,
n-butanol, and aqueous fractions. The antibacterial activity was tested against
two Gram-positive bacteria, viz. Bacillus subtilis (ATCC 6633) and
Staphylococcus aureus (ATCC 25923), and two Gram-negative bacteria, viz.
Escherichia coli (ATCC 25922) and Vibrio cholerae (ATCC 14035) using the agar
disc diffusion assay. Among them, the n-hexane fraction was the most active
against three tested bacteria, viz. Bacillus subtilis, Staphylococcus aureus
and Vibrio cholerae at the concentration 125 µg/ml, with inhibition zone 14.2,
18.2, 13.8 mm, respectively. Isolation and purification of the active component
from the n-hexane fraction led to a known cembranoid-type diterpene,
sarcophytoxide. The chemical structure of the isolated compound was determined
by IR, MS and NMR, as well as compared to data from the literature.
Sarcophytoxide showed moderate activity against B. subtilis, S. aureus and V.
cholerae, with a minimum inhibitory concentration (MIC) of 125, 100, 125 mg/ml,
respectively.
Keywords: antibacterial,
cembranoid, soft coral, Sarcophyton trocheliophorum
Author: Masteria Yunovilsa
Putra, Ahmad Saparhadi, Firmansyah Karim, Tutik Murniasih, Respati Tri Swasono
Journal Code: jpperikanangg160036
