Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate
Abstract: Screening of
alginate lyase producing bacteria associated with seaweed Sargassum
crassifolium was carried out, and isolate S245, identified as Bacillus
megaterium S245 was found to produce high alginate lyase activity. This
research was conducted to evaluate activity of the alginate lyase enzyme at
various pHs, temperatures and substrates. Polymannuronate and polyguluronate
were used to evaluate substrate specificities. Alginate lyase activity was
assayed by analysis of reducing sugar released using the 3,5 dinitrosalicylic
acid (DNS) method. The research showed that the activity of alginate lyase was
optimum at pH of 7.0 and temperature of
45 0C. This enzyme was active for both polymannuronate and polyguluronate susbtrates.
The Vmax and Km of this enzyme for polymannuronate and polyguluronate were 200
unit/ml/min and 79.8 mg/ml for polymannuronate substrate and 27.78 unit/ml/min
and 13.17 mg/ml for polyguluronate substrate. This enzyme showed unique
characteristic in working toward the two substrates.
Keywords: alginate lyase,
seaweed, Bacillus, kinetic activity, Sargassum sp.
Author: Subaryono, Yuwanita
Ardilasari, Rosmawaty Peranginangin, Fransisca Rungkat Zakaria, Maggy
Thenawidjaja Suhartono
Journal Code: jpperikanangg160037
