Investigation on Antioxidant Compounds from Marine Algae Extracts Collected from Binuangeun Coast, Banten, Indonesia
Abstract: Marine algae
contains bioactive secondary metabolites that
have potential to be developed as antioxidant. The aims of this research were to investigate antioxidant
activity and total phenolic compound of marine algae collected from Binuangeun
Beach, Banten and to characterize antioxidant compounds from selected algae
species. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl
radical (DPPH) assay and Ferric Ion Reducing Antioxidant Power (FRAP) Assay,
while total phenolic content was estimated by Folin-Ciocalteau method.
Isolation of bioactive antioxidant compound was performed using SiO2 column
chromatography and preparative high performance liquid chromatography (HPLC).
The antioxidan activity evaluation was conducted to methanol extracts from 20 species (7 Chlorophyta, 9 Phaeophyta
and 4 Rhodophyta). Results showed that Padina australis extract was found to be
the most active. The major bioactive antioxidant compound was identified as fucoxanthin
and a polar compound that was suspected as phenolic compound. The extracts of
P. australis presented the highest phenolic content (58.59 mg GAE/g). A
significant correlation between antioxidant capacity and total phenolic content
was found, indicating that phenolic compounds are the major contributors to the
antioxidant properties of P. australis.
Keywords: marine antioxidant,
DPPH, FRAP, fucoxanthin, phenolic compounds
Author: Muhammad Nursid, Endar
Marasskuranto, Karren Budi Atmojo, Mulyo Pandu Hartono, Maria Diyah Nur
Meinita, Riyanti R
Journal Code: jpperikanangg160035
