POTENSI Rhodococcus pyridinovorans GLB5 SEBAGAI BIOKATALIS DALAM KONVERSI SENYAWA METHIL SIANIDA DAN PHENIL SIANIDA
ABSTRACT: Nitrile and amide
bioconversion have received increasing attention due to their ability to
provide a range of commercially important chemicals. The experiment was
conducted to investigate the potential of bacterial isolate GLB5 to convert
methyl cyanide and phenyl cyanide. The samples were collected from various
industrial waste. Selection of isolates to utilize these substrates as a sole
source of energy, carbon and nitrogen was conducted on 96 whell microtitter
plates, based on the growth ability using INT (Iodo nitrotetrazolium chloride)
reagent. Based on the growth pattern, it showed that the bacterial isolate GLB5
grew well and it was capable of utilizing methyl and phenyl cyanide compound as
the sole source of carbon and nitrogen. The isolate GLB5 was isolated from
industrial waste of Batik factory in Cirebon, and identified as Rhodococcus
pyridinovorans. Bioconversion of methyl cyanide using whole cells of R.
pyridinovorans GLB5 showed that ethanamide (C2H5NO) and ethanoic acid (C2H4O2)
were detected. Formation of ethanamide and ethanoic acid as the product of
bioconversion, indicated that the nitrile hydratase and amidase enzymes
involved in the bioconversion process. Phenyl carboxamide (C7H7NO) as the
product of phenyl cyanide bioconversion was also detected, although in low
concentration. In this study, R. pyridinovorans GLB5 was capable of completely
converting 300 mM methyl cyanide to ± 140 mM ethanoic acid in relatively short
times (<60 minutes).
Key words: bioconversion,
methyl cyanide, phenyl cyanide, Rhodococcus pyridinovorans GLB5,
enzymes-converting nitrile
Penulis: Nunik Sulistinah, Rini Riffiani dan Bambang Sunarko
Kode Jurnal: jpbiologidd160607
