THE EFFECT OF CULTURE MEDIA AND ACTIVATED CHARCOAL ON ASYMBIOTIC SEED GERMINATION AND SEEDLING DEVELOPMENT OF A THREATENED ORCHID Dendrobium taurulinum J.J. Smith IN VITRO
ABSTRACT: Seed germination and
seedling development are an initial and crucial stage in the plant growth and
development. Many factors influence seed and seedling development. The aim of
the present study was to investigate the effects of different culture media (KC,
VW, and MS) and the concentration of activated charcoal (0 g/l and 2 g/l) on
asymbiotic seed germination and seedling development of a threatened orchid,
Dendrobium taurulinum. Results showed that germination occurred regardless of
culture media type, however the percentage of seed germination was higher on
media with 2 g/l activated charcoal (> 90 %) than on media without activated
charcoal (0 g/l). After 12 weeks asymbiotic seed culture, seeds sown on media
with 2 g/l activated charcoal grew and developed more rapidly to leaf-bearing
protocorms (seedlings) compared to media without activated charcoal.The highest
occurrence of advanced seedlings (stage 4, 89.77%) was observed on MS medium
with 2 g/l activated charcoal.
Key words: threatened orchid,
seed germination, seedling development, conservation, Dendrobium taurulinum
Penulis: Siti Nurfadilah
Kode Jurnal: jpbiologidd160606
