Pengaruh Ekstrak Metanol Daun Kayu Kuning (Arcangelisia flava (L.) Merr) terhadap Histopatologi Aorta Tikus Wistar Hiperlipidemia
Abstract: Atherosclerosis is
the leading cause of heart attack and stroke. Hyperlipidemia is one of the
triggers of atherosclerosis through oxidative stress and formation of foam
cells. Atherogenic index (the ratio of LDL/HDL) is a predictive parameter of
coronary heart disease. A.flava is potential as antiaterosklerosis due to the
content of berberine and flavonoids. This study was undertaken to determine the
ability of methanolic extract of A.flava leaves (EMDAf) in lowering atherogenic
index and the number of foam cells. Five groups experimental animals (each
consisting of 4 rats) was induced with high fat and fructose diet for 45 days,
followed by administration of EMDAf dose of 250 mg/kg BW, 500 mg/kg BW, 750
mg/kg BW, simvastatin 1.8 mg/kg BW (control (+)), and CMC Na 1% (control (-))
for 7 days treatments. Then the rat arcus aorta was taken for histopathological
examination with Masson Trichrome staining. ANOVA test results showed that
EMDAf could decrease the atherogenic index, but not significantly (p> 0.05).
While the Kruskall Wallis test results showed that the increase dose of EMDAf
might improve histopathology of the aorta through a reduction in the number of
foam cells significantly compared to the negative control (p <0.05).
Berberine contents in EMDAf is 0.046% measured by TLC-densitometry. It could be
concluded that methanolic extract of A.flava leaves had the ability to decrease
in the atherogenic index value and the number of foam cells.
Penulis: Yuniar Wahyu
Rahmawati, Evi Umayah Ulfa, Ema Rachmawati
Kode Jurnal: jpkesmasdd160088
