Penentuan Kadar Fenol Total pada Ekstrak Daun Tanaman Menggunakan Metode Spektroskopi NIR dan Kemometrik
Abstract: Phenols are group
which having one or more hydroxyl groups which attached in aromatic ring and
are found in plants. The aim of this research was to study whether NIR and
chemometric methods could be used to determine the total phenol content of
leave extracts. These methods were compared to UV-Vis spectrophotometry. Phenol
was extracted from plant leaves by ultrasonic and maceration. NIR spectral data
of selected leave extracts were correlated with total phenol content using
chemometric. In this study, the chemometric method that used for quantitative
and qualitative analysis were Partial Least Square (PLS) and Linear
Discriminant Analysis (LDA), respectively. The PLS R2 calibration was 0.9920369
and RMSEC was 2.0049472. In addition, the R2 of LOOCV and
2-Fold-Cross-Validation were 0.9939401 and 0.9834646, respectively.
Furthermore, LDA gave accuracy of 100%. The significance of phenol total that
have been measured by NIR and UV-Vis spectrophotometry was evaluated with
paired samples T-test and gave no significant difference. In conclusion, total
phenol content can be measured using NIR and chemometric methods.
Penulis: Fracilia Arinda
Ratnasari, Lestyo Wulandari, Nia Kristiningrum
Kode Jurnal: jpkesmasdd160089
