EFEKTIVITAS EKSTRAK BIJI JINTAN HITAM (Nigella sativa Linn.) TERHADAP INFEKSI BAKTERI STREPTOCOCCUS AGALACTIAE PADA IKAN NILA (Oreochromis niloticus Linn.)
ABSTRACT: Black Cumin Seeds
(Nigella sativa L.), commonly known as black seed, have been used in
traditional medicine. Streptococcus agalactiae is a Gram-positive bacteria that
causes the disease Streptococcosis in nile tilapia (Oreochromis niloticus L.).
This study aims to find the bioactivity of black cumin seeds (N. sativa L.)
against bacterial pathogens in nile tilapia (O. niloticus L.). The research activities
include preparation of samples black cumin seeds (N. sativa L.), extraction
with kinetic maceration method using solvents from different polarity (nhexane,
ethanol and methanol), determination of antibacterial activity and minimum
inhibition concentration (MIC) by agar diffusion method. The results of
antibacterial activity against S. agalactiae of pathogenic bacteria showed that
the highest activity was performed by semi-polar extract ethanol (inhibition
zone diameter of 15.33 mm), followed by extract polar methanol with inhibition
zone diameter of 12.67 mm. Extract non-polar nhexane did not show any
activities against the bacterial test. Determination of the minimum inhibitory concentration
(MIC) of ethanol extract against S. agalactiae showed a value of 250 ppm.
Keywords: Antibacteria,
Streptococcus agalactiae, Nile Tilapia (Oreochromis niloticus L.), Nigella
Sativa L
Penulis: Gustiana, Alexander
Rantetondok, Elmi Nurhaidah Zainuddin
Kode Jurnal: jpperikanandd150176
