UJI TOKSISITAS (Brine Shrimp Lethality Test) dan UJI AKTIVITAS ANTIBAKTERI DAUN KEMBANG BULAN (Tithonia diversifolia A. Gray) TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli
Abstrak: Phytochemical,
toxicity tests (Brine Shrimp Lethality Test) and the antibacterial activity of
kembang bulan leaf (Tithonia diversifolia A. Gray). Phytochemical test results
of secondary metabolites kembang bulan leaf (Tithonia diversifolia A. Gray)
show that the ethanol extract contains alkaloids, flavonoids, steroids,
phenolic and saponins. N-hexane fraction containing alkaloids and steroids.
Ethyl acetate fraction contain flavonoids and steroids. Shrimp larvae toxicity
test values obtained Lethal Concentration 50% (LC 50 ) at 383.5660 ppm ethanol
extract, fractions 325.1583 ppm n-hexane and ethyl acetate fraction 196.3755
ppm. Test antibacterial activity against Staphylococcus aureus (Gram positive)
and Escherichia coli (Gram negative) indicates that the most active fraction
was ethanol extract the value of minimum inhibitory 1-2% (w / v) where the
clear zone diameter of 8.5 to 9, 75 mm in the bacterium Escherichia coli and
from 7.5 to 9.75 mm in Staphylococcus aureus.
Penulis: Mira Wahyuni, Chairul
Saleh, Rudi Kartika
Kode Jurnal: jpkimiadd150409
