PRODUKSI GLUKOSA DARI BATANG KELAPA SAWIT MELALUI PROSES HIDROLISIS SECARA ENZIMATIS MENGGUNAKAN AMILASE TERMOSTABIL

ABSTRACT: This  study  aimed  to  explore  the  optimization  of  the  concentration  of  oil  palm  trunks  and thermostable  amylase  enzyme  preformance  produce  highest  sugar  (glucose  total  and  reducing sugars) as well as the degree of polymerization. The design used in this study was a completely randomized  design  (CRD)  factorial  using  2  factors:  factor  A  which  consists  of  four  levels  of amylase  enzyme  that  A1: 250  units /  kg,  A2:  500  units  /  kg,  A3:  750  units  /  kg  and  A4:  1000 units / kg. Factor B is the concentration of oil palm trunk yitu B1: 20% (200 g / 1 liter) B2: 40% (400  g  /  1  liter)  and  B3:  60%  (600  g  /  1  liter)  were  repeated  3  times.  The  results  showed  that there  is  a  highly  significant  interaction  (P  <0.01)  in  the  levels  of  amylase  enzyme  (factor  A) with the concentration of oil palm trunks (factor B) on reducing sugar, siqnificantly interaction (P  <0.05)  on  total  sugars  and  degree of  polymerization  (DP)  . This  research can  be  concluded that the best treatment at a concentration of 60% oil palm trunks and levels  250 unit/kg of an enzyme  concentration  that  produces  of  total  sugars  7.86  mg/ml,  reducing  sugar  15.69  mg  /ml and degree of polymerization 0.50
Keywords: amilase, total gula, gula pereduksi, derajat polimerisasi
Penulis: Yetti Marlida, Mirzah, Syukri Arief dan  Khoirul Amru
Kode Jurnal: jpkimiadd140717

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