PENGARUH INTENSITAS CAHAYA TERHADAP BIOMASSA DAN KADAR SAPONIN KALUS GINSENG JAWA (Talinum paniculatum Gaertn.) PADA BERBAGAI WAKTU KULTUR
Abstract: The aims
of this research
was to study
the effects of
light intensity on
callus biomass and saponin
content of ginseng
jawa (Talinum paniculatum
Gaertn.). Callus induced from leaf explants on solid Murashige and Skoog (MS) medium supplemented by
2 mg/L of
2,4-D and 1
mg/L of kinetin.
Callus induced by treatment
of combination between
light intensities 1000,
2000, 4000, 6000
lux, also dark condition
and culture period
4, 6, and
8 weeks. Each
treatment of combination was
repeated 6 times.
Retrieval of data
were callus biomass
(fresh and dry weight)
and saponin content.
The data of
biomass were analyzed
by using manova test
(significance of 5%).
Saponin content was
analyzed semi-quantity using
Thin Layer Chromatography (TLC).
The result showed
that the highest average
of dry weight
(as parameter) obtained
at treatment combination 4000 lux
light intensity and 8 weeks culture period was 0,0699 gram. Meanwhile the highest
saponin content obtained
at 4000 lux
light intensity and
6 weeks culture period was 58,5
mm2/0,01 gram dry weight of callus and color intensity +4. With
the results that light intensities influence on callus biomass and saponin content
of ginseng jawa at different culture period.
Keywords: biomass,
callus, culture period,
light intensity, saponin
content, Talinum paniculatum Gaertn
Penulis: Afifatul Alwiyah, Y.
Sri Wulan Manuhara, dan Edy Setiti Wida Utami
Kode Jurnal: jpbiologidd150378
