PENENTUAN ZAT PEMANIS BUATAN PADA SAMPEL MINUMAN OLAHAN DI LINGKUNGAN SEKOLAH DASAR SE-KECAMATAN PAUH, KOTA PADANG SECARA KROMATOGRAFI CAIR KINERJA TINGGI (KCKT)
Abstract: Determination of
Artificial Sweeteners Substances
at Beverages Samples
in Elementary School Environmental in
throughout Pauh, Padang
city with High
Performance Liquid Chromatography (HPLC)
has been done.
This study aimed
to determine the
levels of artificial sweeteners in
beverages processed in the school
environment whether contents
is still legal compliance. Condition
of the research
were reverse phase
of HPLC method
used C18 column (150 mm x 4.6 mm), mobile phase
aquabidest : acetonitrile (95: 5), flow rate 1 mL/minute with the UV detector λ
220 nm. The determination coefficient of sodium saccharin was 0,996 whereas sodium
cyclamate was 0.964. The analysis showed all the samples containing sodium
saccharin but not detected sodium cyclamate. Sodium saccharin content of the A,
B, C, D, E, F, G, and H samples 32.8995 mg, mg 30.0642, 59.2972 mg, mg 35.8975,
21.8812 mg, 46.0881 mg, mg 60.2040, and
75.6120 mg, respectively.
Sodium saccharin content
are still within
the limits allowed, except in sample H had crossed the
permissible limit maximum content of sodium saccharin in beverages according to
SNI 01-0222-1995 is 0 to 2.5 mg / kg.
Penulis: Yulia Rani Putri,
Zulfarman, dan Zamzibar Zuki
Kode Jurnal: jpkimiadd150279
