ISOLASI DAN KARAKTERISASI SENYAWA KUMARIN DARI EKSTRAK AKTIF ETIL ASETAT KULIT BATANG LOA (Ficus racemosa L) SEBAGAI ANTIOKSIDAN
Abstract: Isolation of
coumarin from the
bark of Loa
(Ficus racemosa L)
has been performed.
Isolation by maceration method
using n-hexane and ethyl acetate as solvent. Antioxydant ability is measured by
the method of using the free radical DPPH (1,1 -diphenyl-2-picrylhydrazyl) and
it shows both the active extract
has a good
capacity as antioxydant.
Ethyl acetate extraction
used very actived as antioxydant and IC50 value was 29
651 mg / L, and the ethyl acetate extract was separated by column
chromatography (stationary phase: silica and mobile phase n-hexane: ethyl
acetate (4: 6) with isocratic method.
insulating compound is
a yellowish white
amorphous (3mg) and decompotitioned at
185oC, and it
resulted in a
single place with
blue fluorisence at
λ 365 nm. Identification used coumarin-specific
reagent (1% NaOH, NH4OH) causes fluorisence of becamed pale blue dark blue.
Coumarin it do not activate it as antioxydant with IC50 value 516.5 mg / L.
Penulis: Adlis Santoni, Mai
Efdi dan Elza Prima Sari
Kode Jurnal: jpkimiadd150279
