ISOLASI DAN KARAKTERISASI SENYAWA KUMARIN DARI EKSTRAK ETIL ASETAT DAUN TANAMAN MURBEI (Morus alba L)
Abstract: Secondary
metabolites of coumarin compound has been isolated from Mulberry’s leaves
(Morus alba L). The powder of leaves was extracted by maceration method using
hexane, ethyl acetate, and methanol as
solvent. The isolated
compound has the
shape of yellowish-white powder, melts at 125-1260C and based on test
on TLC given single spot that blue fluorescent under UV light at
λ 365 nm.
Based on the
UV and IR
spectrum of this
compound, it showed
absorption chromophore of lactone ring and has a functional group O-H at 3431.87 cm-1, C-H stretching at
2924.48 cm-1, C=O stretching at 1718.93 cm-1, C=C stretching 1624.30 cm-1 and
C-O stretching at 1263.84 cm-1. The toxicity test with the method of brine
shrimps test showed that the fraction of
hexane, ethyl acetate
and methanol has
toxicity activity. Ethyl
acetate extract has
a high toxicity with LC50 134.152
mg/L.
Penulis: Mico Diotoma,
Hasnirwan, Djaswir Darwis
Kode Jurnal: jpkimiadd150279
