ISOLASI DAN KARAKTERISASI SENYAWA METABOLIT SEKUNDER DARI FRAKSI AKTIF DAUN TEMPUYUNG (Sonchus arvensis L.) TERHADAP UJI TOKSISITAS
Abstract: Isolation of
secondary metabolite compound
from tempuyung leaves
(Sonchus arvensis L.)
has been done. Toxicity
assay of n-hexane
extract, ethyl acetate
extract, methanol extract
and water extract has been
tested, and methanol extract obtained high activity in toxicity assay using
Brine Shrimps Lethality Test
(BSLT) with LC50
values 403.645 mg/L.
Isolation was continued
by maceration using methanol
solvent. Methanol extract
was soluted with
water and than
was fractionated with n-hexane
and has been
continued by ethyl
acetate solvent. Toxicity
of each fractions was tested, and
water fraction obtained highest activity with LC50 values 269.774 mg/L. Water
fraction was freeze-dried and column chromatographied using silica gel as the stationary phase and
n-hexane, ethyl acetate
and methanol as
the mobile phase
with using Step
Gradient Polarity (SGP) method.
Isolated compound is
white-brown solid around
3 mg which
has single spot with
some eluent comparisons
on thin layer
chromatography. The results
of chemical characterization of
isolated compound using
spectroscopy method indicates
that the isolated compound was included as coumarin
group.
Penulis: Arrijal Mustakim,
Afrizal, dan Mai Efdi
Kode Jurnal: jpkimiadd150280
