ISOLASI DAN KARAKTERISASI SENYAWA KUMARIN DARI KULIT BATANG Fagraea ceilanica Thunb. SERTA UJI ANTIOKSIDAN
Abstract: Isolation of
coumarin compounds from
the bark of
Fagraea ceilanica Thunb.
have done by maceration method
using a solvent of n-hexane, ethyl acetate and methanol. A silica gel column was used
for the chromatographic isolation
of the ethyl
acetate extract components.
N-hexane and ethyl acetate as the mobile phase in the SGP (Step Gradient
Polarity). Isolated compounds in the form of dark yellow crystals as much as 26
mg which has a melting point of 205 - 207ºC. It gave a single spot in TLC (Thin
Layer Chromatography) (Rf 0.42) which eludated with mixture of n-hexane
: ethyl acetate
(7:3). It fluorescent
under 365 nm
UV and become
brighter after sprayed with 5%
NaOH as the specific reagent for coumarin. Based on the UV and IR spectrum of this
compound showed absorption
chromophore lactone ring
and has a
functional group -OH at 3318 cm-1, C-H stretching at 2926
cm-1, C = O stretching at 1698 cm-1, C = C stretching at 1602 cm-1, and C-O
stretching at 1263 cm-1 which indicates that the isolated compounds such as coumarin group
which has the
-OH group. Test
results antioxidants with
DPPH free radical method shows
methanol and ethyl
acetate extracts were
moderate to its
activity as an antioxidant which has IC50 values
respectively - also at 118.76 mg/L and 175.68 mg/L, whereas the n-hexane
extract inactive with
IC50 value of
6524.67 mg/L. Isolated
compounds also has potential as a relatively weak
antioxidant with IC50 value of 358.71 mg/L.
Penulis: Rizki Alfajri, Norman
Ferdinal, dan Bustanul Arifin
Kode Jurnal: jpkimiadd150279
