ISOLASI DAN KARAKTERISASI SENYAWA TRITERPENOID SERTA UJI ANTIBAKTERI PADA DAUN Ficus variegata Blume
Abstract: Isolation of
triterpenoid compound from the leaves of Ficus variegata Blume have been done
by maceration method using
n-hexane solvent. N-hexane
extract was chromatographed column using
a silica gel
as the static
phase, n-hexane and
ethyl acetate 8
: 2 as
the mobile phase
in a Isocratic manner. Isolated compounds resemble the
white needle crystals with a melting point in 136º
- 138ºC. TLC
test (Thin Layer
Chromatography) gave a
purple single stain
using Liebermann-Burchard reagent with Rf 0.5 which not fluorescence
under UV rays at λ 254 nm and 365
nm. Based on
UV and IR
spectrum this compound
indicates presence of
double bond which not
conjugated at 212,14
nm and having
a functional group
–OH at 3439,39
cm-1, C-H stretching at
2935,69 cm-1, C=O
stretching at 1710,57
cm-1, C=C stretching
at 1638.34 cm-1,
C-O stretching at 1051.83 cm-1, 1376.76 cm-1 and 1466.05 cm-1 are geminal dimethyl group (C-(CH3)2) which
indicates that the isolated compound is
triterpenoid. The result of antibacterial test using diffusion test
in order to
show medium active
of n-hexane extract
at concentration 20
mg/mL with hallozone 7.32 mm as antibacterial against bacteria of Staphylococcus aureus and not active in
Escherichia coli bacteria.
Penulis: M. Iqbal, Adlis
Santoni, Mai Efdi
Kode Jurnal: jpkimiadd150281
