Isolasi dan Uji Bioaktivitas Senyawa Metabolit Sekunder Ekstrak N-Heksan Daun Pare (Momordica charantia L.)
Abstract: Bittermelon
(Momordica charantia L.) is a bioactivity plant that used as a medicine
traditional. The purpose of this research is to isolate the bioactivity
secondary metabolites of n-hexane extract from the bittermelon leaves and
bioassay toward larval shrimp A. salina. Isolation of the secondary metabolites
including extraction, fractionation, purification, and identification. Bioassay
was conducted to the extract and to mixed fractions (fraction A – G) the
results of column chromatography. The results of research showed that the
extracts and fractions from bittermelon leaves was toxic to A. salina. The LC50
from extracts bittermelon leaves was 91, 84 ppm. The LC50 for the fractions A
and B (steroid) were 38,26 ppm and 85,90
ppm, the fractions C – G (terpenoid) were 94,45 ppm; 79,56 ppm; 92,53 ppm;
70,41 ppm; and 92,69 ppm, the most toxic fractions was fraction of A. From the
C-fraction as a green coulor was obtained a crystal and needle-shaped. After
recystalization with n-hexane and ethylacetat was obtained a pure compound that
positive to terpenoids with Liebermann-Burchard reagents.
Penulis: Muharram
Kode Jurnal: jpbiologidd100186
