THE INFLUENCE OF POLYVINYL PYRROLIDONE (PVP) ON PIROXICAM ABSORPTION WITH EVERTED INTESTINAL SAC METHOD
ABSTRACT: Oral drug absorption
was highly influenced by dissolution
rate, especially for poorly and insoluble drugs. Piroxicam is a nonsteroidal
anti-inflammatory drug that is practically insoluble in water. The oral
absorption rate of piroxicam is dependent on its dissolution rate in the GI
tract. Polyvinyl Pyrrolidone (PVP) as surfactant can increase drug solubility
by means of a micelle forming mechanism. The aim of the study was to know the
influence of addition and variation of Polyvinyl Pyrrolidone (PVP) on Piroxicam
absorption with everted intestinal sac method.
The concentration of piroxicam solution was prepared by PVP in 1.0, 2.0 and 3.0 % respectively. Crane
and Wilson tube
containing 75 ml of
the mucosal fluid was taken at 370C in waterbath.
Than, serosal solution of 1.5
ml was added to intestinal sac by turned upside down and tied to a
cannula, then put into the tube
containing the mucosal fluid
and constantly flowing oxygen gas. Serosal solution of 1 ml
were taken every 15 minutes and then diluted with 2 ml of Ba (OH) 2 and 2 ml of
ZnSO4 then centrifuge until 25 minute. The absorbant of supernatant was measured
by UV spectrophotometer and data analyse was calculated by one-way
ANAVA. PVP at 1%,2% and 3% increased
piroxicam absorption from the phosphate buffer pH 7.5 compared with negative
control. According to Papp, the values were 2.52 ± 0.43 cm/minute (negative
control), 3.41 ± 2.17 cm/minute (1% PVP),
2.75 ± 1.14 cm/minute (2% PVP)
and 4.77
± 4.93 cm/minute (3% PVP) respectively. In conclusion. Lower doses of
the surfactant (1%, 2%, and 3% PVP) significantly increased absorption of the
drug by altering the membrane permeability.
Author: Fea Prihapsara. Anif
Nur Artanti, Mimiek Murrukmihadi
Journal Code: jpfarmasigg150023
