ANTIBACTERIAL ACTIVITY OF Chromolaena odorata (L) King LEAVES WITH BIOAUTOGRAPHY
ABSTRACT: Chromolaena odorata
(L) King, commonly referred as kirinyuh, is a traditional medicinal plant from
Asteraceae. It has been reported that C. odorata scientifically possessed antimicrobial
activity. The aim of this study is to obtain active antibacterial substances from
70% etanolic extract of C. odorata against Staphylococcus aureus. Separation of
active substances was carried out by Thin Layer Chromatography (TLC)
techniques. The separated substances were tested for their antibacterial
activity by bioautography assay using S. aureus. Separation of 70% etanolic
extract C. odorata produced six spots with retention factor (Rf) values are
0.9, 0.8, 0.7, 0.6, 0.5, and 0.3. The diameters of inhibition zone of those
spots were 35, 27, 27, 20, 31, and 14 mm, respectively. Based on the TLC profiles,
the compounds with Rf of 0.9, 0.6, and 0.5 were identified as flavonoids and
the compounds with Rf of 0.8, 0.7, and 0.3 were identified as terpenoids. It is
concluded that the spot with the most potent antimicrobial activity was
flavonoids with of Rf 0.9.
Author: Siti Rofida,
Nurwahdaniati
Journal Code: jpfarmasigg150022
